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CD20, a 33-36 kDa non-glycosylated type 1 transmembrane protein, is expressed by developing, resting and mature B cells, some follicular dendritic cells, and a small subset of mature T cells. B cell CD20 expression is lost upon differentiation into plasma cells. Functionally, CD20 activation contributes to B cell activation, proliferation, and differentiation.
Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5μg (unconjugated, Biotin) or 5μl (conjugated) of these products are sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Non-human primate cross-reactivity has been validated using Caprico’s PerCP conjugated clone 2H7 product. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].