The monoclonal UCHT1 clone recognizes CD3? antigen, a 20KDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. The CD3 complex contains a CD3? chain, a CD3? chain, and two CD3? chains. These chains associate with a molecule known as the T-cell receptor (TCR) and the ?-chain (zeta-chain) to generate an activation signal in T lymphocytes. CD3? is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays central roles in TCR signaling, T lymphocyte activation, and antigen recognition. Crosslinking of the TCR via plate bound UCHT1 monoclonal is widely used to study the activation T cell response in vitro.
mFluor™ fluorescent labeling dyes are developed specifically for flow cytometry applications to enable optimal multi-color detection. These dyes are excellent alternatives to the phycoprotein-based tandems that are quite difficult to couple to an antibody or other biomolecules and offer greater light stability and brighter fluorescence. The mFluor™ 450 fluorophore is a great alternative to these dyes: V450, Pacific Blue™, VioBlue?, eFluor? 450, Brilliant Violet 421 and Super Bright 436. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].